World No.19 Nick Kyrgios suffered an unexpected second-round loss to Argentina's Nicolas Kicker at the Lyon Open.
The Australian No. 4 seed, given a bye in the first round, took the opening set before eventually going down 2-6, 6-4, 6-2 against Kicker, who is ranked at 94.
There was, though, no such drama for No. 6 seed Juan Martin Del Potro in his first-round match as the Argentinian coasted past Quentin Halys 7-5, 6-4.
Elsewhere in the second round, Nikoloz Basilashvili beat Italian Andreas Seppi 6-4, 6-4 and the Georgian will next meet Kicker in the quarter-finals.
Earlier on Tuesday, Kyle Edmund battled into the second round with a 2-6, 6-4, 6-1 victory over Brazilian Thiago Monteiro.
The Briton will play home favourite Gilles Simon for a place in the last eight. Russian Karen Khachanov earned his place in the second round with a straight-sets 6-2, 6-4 win against Renzo Olivo of Argentina.
Carlos Berlocq also progressed with a 6-2, 6-4 success over American Tennys Sandgren.